Increase in local protein concentration by field-inversion gel electrophoresis |
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| Authors: | Henghang Tsai Teck Yew Low Steve Freeby Aran Paulus Kalpana Ramnarayanan Chung-pui Paul Cheng Hon-chiu Eastwood Leung |
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| Institution: | (1) Medical Proteomics and Bioanalysis Section, Genome Institute of Singapore, Singapore;(2) Bio-Rad Laboratories, Hercules, USA;(3) Department of Molecular and Cellular Biology, Department of Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston, USA |
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| Abstract: | Background Proteins that migrate through cross-linked polyacrylamide gels (PAGs) under the influence of a constant electric field experience
negative factors, such as diffusion and non-specific trapping in the gel matrix. These negative factors reduce protein concentrations
within a defined gel volume with increasing migration distance and, therefore, decrease protein separation efficiency. Enhancement
of protein separation efficiency was investigated by implementing pulsed field-inversion gel electrophoresis (FIGE). |
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