Discovery and identification of Serum Amyloid A protein elevated in lung cancer serum |
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Authors: | Dai SongWei Wang XiaoMin Liu LiYun Liu JiFu Wu ShanShan Huang LingYun Xiao XueYuan He DaCheng |
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Institution: | 1.Key Laboratory of Cell Proliferation and Regulation of Ministry of Education,Beijing Normal University,Beijing,China;2.Universities’ Confederated Institute of Proteomics,Beijing,China;3.Department of Thoracic Surgery,General Hospital of Beijing Unit, PLA,Beijing,China |
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Abstract: | Two hundred and eighteen serum samples from 175 lung cancer patients and 43 healthy individuals were analyzed by using Surface
Enhaced Laser Desorption/Ionization Time of Flight Mass Spectrome-try (SELDI-TOF-MS). The data analyzed by both Biomarker
Wizard™ and Biomarker Patterns™ software showed that a protein peak with the molecular weight of 11.6 kDa significantly increased
in lung cancer. Meanwhile, the level of this biomarker was progressively increased with the clinical stages of lung cancer.
The candidate biomarker was then obtained from tricine one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis
by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein (SAA) by MALDI/MS-MS
and database searching. It was further validated in the same serum samples by immunoprecipitation with commercial SAA antibody.
To confirm the SAA differential expression in lung cancer patients, the same set of serum samples was measured by ELISA assay.
The result showed that at the cutoff point 0.446 (OD value) on the Receiver Operating Characteristic (ROC) curve, SAA could
better discriminate lung cancer from healthy individuals with sensitivity of 84.1% and specificity of 80%. These findings
demonstrated that SAA could be characterized as a biomarker related to pathological stages of lung cancer. |
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Keywords: | lung cancer Serum Amyloid A protein Surface Enhanced Laser Desorption/lonizaion Time of Flight Mass Spectrometry |
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