Stoichiometry of elongation factor G function in translation |
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Authors: | A A Dahlfors C G Kurland |
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Affiliation: | Department of Molecular Biology, Uppsala University, Sweden. |
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Abstract: | A steady-state translation system has been used in vitro to measure the stoichiometry with which elongation factor G-GTP complexes are dissipated during polypeptide elongation. It has been possible to separate this dissipation from that associated with elongation factor Tu function. Our measurements for the wild-type as well as for two mutant variants indicate that there is one elongation factor G-GTP complex dissipated per peptide bond in the steady-state. |
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