Studies on isolated mitochondria from muscles of normal and dystrophic chickens. |
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| Authors: | J H Thakar |
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| Affiliation: | 1. Department of Physiology, University of Veterinary Sciences Brno, Brno, Czech Republic;2. Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic;3. University of Veterinary Medicine, Vienna Department of Biological Sciences and Pathobiology Centre of Biological Sciences;4. Department of Orthopedic Surgery, Experimental Orthopedics, Centre for Medical Biotechnology (ZMB), University of Regensburg, Biopark 1, Germany;1. Department of Gynecology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China;2. Department of Andrology, Xuzhou Hospital Affiliated to Nanjing University of Chinese Medicine, Xuzhou, China;3. Andrology Department, Wenzhou City Hospital of traditional Chinese medicine and Western medicine combined, Wenzhou, China;4. Andrology Department, Cixi integrated Traditional Chinese and Western Medicine Medical & Health Group, Cixi, China;5. Andrology Department, Ningbo Hospital of Traditional Chinese Medicine, Ningbo, China;6. Department of Urology Surgery, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, China;7. College of Traditional Chinese Medicine of Tianjin University of Traditional Chinese Medicine, Tianjin, China;8. Department of Nephrology, Chongqing Hospital of Traditional Chinese Medicine, China;1. Department of Anatomy, University of Ilorin, Ilorin, Nigeria;2. Department of Physiology, University of Ilorin, Ilorin, Nigeria;3. Department of Anatomy, Al-Hikmah University, Nigeria;4. Department of Obstetrics & Gynaecology, University of Ilorin Teaching Hospital, Nigeria |
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| Abstract: | Oxidative phosphorylation was studied in mitochondria from different fiber types in normal (line 412) and dystrophic (line 413) chickens in vitro. Three types of muscles, namely, superficial pectoralis (αW), lateral adductor (βR), and medial adductor (αR) were used. Mitochondria from adductor muscles of both normal and dystrophic chickens equally utilized a variety of substrates such as pyruvate-malate, succinate, β-hydroxybutyrate, and l-glutamate with the coupled phosphorylation of ADP. These organelles did not oxidize α-glycerophosphate with coupled ATP synthesis. However, the organelles from pectoralis muscles oxidized α-glycerophosphate and oxygen consumption in the presence of this substrate was repeatedly stimulated by the addition of limited quantities of ADP. The rate of oxidation of this substrate was significantly lower in dystrophic mitochondria as compared to normal organelles. Although, the organelles from dystrophic pectoralis muscles oxidized pyruvate-malate, succinate, and β-hydroxybutyrate with the same efficiency as normal muscle mitochondria, the rate of l-glutamate oxidation was lower than normal. All the isolated organelles oxidized NADH at a very slow rate and this oxidation was not coupled with the phosphorylation of ADP. Unlike results previously reported by many investigators, there was no difference in the yield of mitochondria from dystrophic muscle, but the cytochrome oxidase activity of dystrophic muscle homogenates was higher than that of normal muscle. These data strongly suggest the existence of an abnormality in the NADH “shuttle systems” of dystrophic pectoralis muscle mitochondria. |
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