| 大鼠肝癌细胞中失活的肿瘤相关基因的克隆和筛选 |
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| 引用本文： | 史璨,胡拥军,徐链,王珮.大鼠肝癌细胞中失活的肿瘤相关基因的克隆和筛选[J].分子细胞生物学报,1994(4). |
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| 作者姓名： | 史璨 胡拥军 徐链 王珮 |
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| 作者单位： | 中国科学院上海细胞生物学研究所分子肿瘤学研究室 200031
(史璨，胡拥军，徐链)，中国科学院上海细胞生物学研究所分子肿瘤学研究室 200031(王珮) |
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| 基金项目： | 中国科学院上海细胞生物学研究所所长择优基金 |
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| 摘 要： | 本文描述了运用减式杂交技术筛选只在大鼠正常肝细胞中特异性表达,而在肝癌细胞中失活的肿瘤相关基因的实验。实验中用肝癌细胞mRNA杂交筛减正常肝细胞cDNA得到探针A,用正常肝细胞mRNA杂交筛减肝癌细胞cDNA得到探针B。文章描述了用这两种探针对2500个重组子点杂交筛选得到的与探针A杂交强的cDNA克隆中的6个:TR1-6,并主要分析了其中两个在正常肝细胞中特异性表达的情况。实验中还建立了cDNA库,以用于体外转录mRNA及今后的全长cDNA筛选。
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| 关 键 词： | 肿瘤相关基因 减式杂交 TR系列 cDNA |
CLONING AND SCREENING ON TUMOR-RELATED GENES INACTIVATED IN RAT HEPATOMA CELLS |
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| Shi Can Hu Yong-jun Xu Lian Wang Pei.CLONING AND SCREENING ON TUMOR-RELATED GENES INACTIVATED IN RAT HEPATOMA CELLS[J].Journal of Molecular Cell Biology,1994(4). |
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| Authors: | Shi Can Hu Yong-jun Xu Lian Wang Pei |
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| Abstract: | The paper describes an experiment screening tumor-related genes which specifically expressed in rat normal liver cells but inactivated in hepatoma cells with subtractive hybridization technique. During the test, subtracting normal liver cD-NA with hepatoma mRNA produces probe -A whereas subtracting hepatoma cDNA
by normal liver mRNA gives probe-B. The paper shows six cDNA fragments(TR 1-6) which hybridize probe-A preferentially obtained through dot-blot screening of 2500 colonies with the two probes. Among TR 1-6, special expressions of TR 2 and TR 4 in normal liver cells are further analyzed. |
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| Keywords: | Tumor-related genes Subtractive hybridization cDNA TR 1-6 |
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