The histone H5 variant in Xenopus laevis |
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| Authors: | A F Moorman P A de Boer M T Linders R Charles |
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| Affiliation: | 1. Departments of Orthopaedic Surgery and Medicine, Center for Research in FOP & Related Disorders, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States;2. Department of Pediatrics, Division of Hematology-Oncology, University of Rochester School of Medicine, Rochester, NY 14642, United States;3. Division of Oncology, Department of Pediatrics, The Children''s Hospital of Philadelphia, Philadelphia, PA 19104, United States;4. Department of Pediatrics, Division of Pediatric Hematology-Oncology, Jonathan Jacques Children''s Cancer Center, Long Beach, CA 90806, United States;5. Department of Pediatrics, Division of Hematology-Oncology, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, United States;6. Lady Cilento Children''s Hospital, Rheumatology Department, South Brisbane 4101, Australia;7. Department of Paediatrics, the Royal National Orthopaedic Hospital, Stanmore, Middlesex HA7 4LP, United Kingdom;8. Division of Pediatric Rheumatology, Department of Pediatrics, University of Rochester School of Medicine, Rochester, NY 14642, United States;9. Department of Rheumatology, the Royal National Orthopaedic Hospital, Stanmore, Middlesex HA7 4LP, United Kingdom;10. Division of Endocrinology and Metabolism, Department of Medicine, Institute for Human Genetics, University of California-San Francisco School of Medicine, San Francisco, CA 94143, United States;11. Department of Medicine, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN 55905, United States;1. School of Science, The University of Waikato, Private Bag 3105, Hamilton, New Zealand;2. National Institute of Water and Atmospheric Research Ltd. (NIWA), PO Box 11-115, Hamilton 3200, New Zealand;1. School of Environment, Harbin Institute of Technology, Harbin 150090, China;2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;3. Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, China |
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| Abstract: | The presumptive histone H5 of Xenopus laevis has been characterized by SDS and acid-urea-Triton polyacrylamide gel electrophoresis and compared with chicken histone H5. Chicken H5 has a lower electrophoretic mobility compared to that of Xenopus H5 in both gel systems. It is shown, using a polyclonal antiserum against chicken H5, that the Xenopus histone H5 is immunologically related to chicken histone H5. Monoclonal antibodies have been prepared to the Xenopus histone types H5 and H1A, that do not cross-react, as determined by their reactivity in an enzyme linked immunosorbent assay and by their ability to react with either H1A or H5 in an immunochemical test on total erythrocyte histones that are transferred to nitrocellulose after fractionation by SDS- or acid-urea polyacrylamide gel electrophoresis. As all nuclei of erythrocytes from adult Xenopus laevis can be shown to contain histone H1A and H5, these monoclonal antibodies can be used to further delineate the role of H5 in tissue differentiation. |
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