Observation of the phosphatidyl ethanolamine amino proton magnetic resonance in phospholipid vesicles: inside/outside ratios and proton transport. |
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Authors: | Y Lange E K Ralph A G Redfield |
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Affiliation: | Biophysical Laboratories, Harvard Medical School, Boston, Massachusetts 02115 USA;Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02154 USA |
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Abstract: | The amino proton resonance of phosphatidyl ethanolamine in sonicated mixed phospholipid vesicles is observed 3.3 ppm downfield from H2O. Above pH ~ 5 it is broadened beyond detectability as a result of exchange with H2O protons. In low salt, resonances of amino protons inside the vesicles appear to persist as the pH is raised, while those on the outside disappear. Solvent catalized proton conduction along the surface is proposed, with an effective -NH2 to -NH3 transfer rate of about 8 × 105 sec?1 at 25°C. |
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