Lymphatic absorption and transport of retinol and vitamin D-3 from rat intestine Evidence for different pathways |
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Authors: | Rune Blomhoff Per Helgerud Svein Dueland Trond Berg Jan I Pedersen Kaare R Norum Christian A Drevon |
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Institution: | 1. Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, Oslo 3 Norway;2. Department of Pharmacology, Institute of Pharmacy, University of Oslo, Blindern, Oslo 3 Norway |
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Abstract: | The lymphatic absorption and transport of retinol and vitamin D-3 from rat intestine has been studied. When rats were cannulated in the intestinal lymph duct and given an intraduodenal bolus of 3H]retinol and 14C-labelled vitamin D-3, 14C-labeled vitamin D-3 appeared later in the intestinal lymph than 3H]retinol and the rate of absorption of vitamin D-3 was still maximal at a time when that of retinol had declined. Both vitamins were absorbed via the lymphatic route in association with chylomicrons. Almost all the retinol was esterified, while vitamin D-3 appeared in the chylomicrons as free vitamin D-3. In vitro incubations and in vivo studies using hepatectomized and normal rats showed that the retinyl ester was a relatively nonexchangeable component of the chylomicrons and their remnants. Hence, all the vitamin A followed the remnants in their clearance from plasma. In contrast, significant amounts of vitamin D-3 were transferred from the chylomicrons to other plasma fractions. Therefore, only a fraction of this vitamin may be removed in association with the chylomicron remnants. |
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Keywords: | Retinol Vitamin D Chylomicron Lymphatic transport (Rat intestine) HPLC high-performance liquid chromatography I U international units LDL low density lipoproteins HDL high density lipoproteins |
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