Interaction of influenza virus proteins with planar bilayer lipid membranes II. Effects of rimantadine and amantadine |
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Authors: | Vsevolod A. Tverdislov Salam El Karadaghi Doris J. Bucher Juri A. Zakomirdin Igor G. Kharitonenkov |
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Affiliation: | 1. Physical Department of Moscow State University, Moscow U.S.S.R.;2. Department of Microbiology, Mount Sinai School of Medicine of The City University of New York, I Gustave L. Levy Place, New York, NY 10029 U.S.A.;3. Ivanovskii Institute of Virology, Gamaleya St. 16, Moscow U.S.S.R. |
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Abstract: | The dependence of the surface potential difference (ΔU), transversal elasticity module (E1) and membrane conductivity (G0) on the concentrations of the antiviral drugs, rimantadine and amantadine was studied in the planar bilayer lipid membrane system. The method used was based on independent measurements of the second and third harmonics of the membrane capacitance current. The binding constants of bilayer lipid membranes obtained from the drug adsorption isotherms were 2.1 · 105 M?1 and 1.3 · 104 M?1 for rimantadine and amantadine, respectively. The changes in G0 took place only after drug adsorption saturation had been achieved. The influence of rimantadine and amantadine on the interaction of bilayer lipid membranes with matrix protein from influenza virus was also investigated. The presence of 70 μg/ml rimantadine in the bathing solution resulted in an increase in the concentration of M-protein at which the adsorption and conductance changes were observed. The effects of amantadine were similar to those of rimantadine but required a higher critical concentration of amantadine. The results obtained suggest that the antiviral properties of rimantadine and amantadine may be related to the interaction of these drugs with the cell membrane, which can affect virus penetration into the cell as well as maturation of the viral particle at the cell membrane. |
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Keywords: | Influenza virus protein Membrane protein interaction Amantadine, Rimantadine Antiviral agents Surface potential (Lipid bilayer) |
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