An investigation on the effect of oligomycin on state-4 respiration in isolated rat-liver mitochondria |
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Authors: | Alberto Masini Daniela Ceccarelli-Stanzani Umberto Muscatello |
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Institution: | 1. Institute of Biological Chemistry, University of Modena, Via Campi, 287, 41100 Modena Italy;2. Institute of General Pathology, University of Modena, Via Campi, 287, 41100 Modena Italy |
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Abstract: | The inhibitory action of oligomycin on State-4 respiration in rat-liver mitochondria has been investigated in detail with regard to the extent, mode and characteristics of the inhibition. The possibility that this effect may be due either to some damage of the mitochondrial preparation used or to the presence of heavy contaminations by microsomes has been excluded. It has been found that the concentration of specific binding sites is the same in State 4 as in State 3. The extent of the inhibition appears to be related to the ADP concentration, rather than to ratios. The inhibition of this antibiotic on State-4 respiration does not depend on the experimental conditions used (i.e., choice of substrates or composition of the reaction medium). In agreement with these observations, it has been found that the membrane potential of State 4 is significantly increased when oligomycin is added. All these results provide further evidence to the conclusion that a large portion of State-4 respiration is linked to phosphorylation. |
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Keywords: | Oligomycin Respiration Membrane potential ATP synthesis (Rat-liver mitochondria) FCCP DNP 2 4-dinitrophenol |
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