The distribution of ( along the villus crypt-axis in the rabbit small intestine |
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Authors: | Pamela JE Rowling Francisco V Sepúlveda |
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Institution: | A.R.C. Institute of Animal Physiology, Babraham, Cambridge CB2 4AT U.K. |
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Abstract: | The migration of intestinal epithelial cells from the crypts to the tips of villi is associated with progressive cell differentiation. The changes in Na+-pump levels during migration have been measured in epithelial cells isolated from rabbit small intestine. A significant proportion of ouabain-sensitive ( in the cell homogenates was latent but could be unmasked by detergent treatment. Highest detergent activation was observed in villus cells. The distribution of pumping sites was also assessed by measuring ouabain binding to intact cells. The kinetics of specific binding was consistent with the interaction of the cardiac glycoside with a single population of binding sites with an apparent of around 10?7 M. Both enzyme assay and ouabain-binding measurements suggest that a 2–3-fold increase in the number of Na+-pumping sites accompanies cell differentiation in rabbit jejunal epithelium. This increase in pumping capacity might be an adaptation of the cells to their absorptive function. |
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Keywords: | Ouabain binding Differentiation Cell migration (Rabbit small intestine) Hepes To whom correspondence should be addressed |
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