Leakage-channel conductance of single (Na+ + K+)-ATPase molecules incorporated into planar bilayers by fusion of liposomes |
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| Authors: | R. Reinhardt B. Lindemann B.M. Anner |
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| Affiliation: | 1. II. Physiologisches Institut der Universität des Saarlandes, D-6650 Homburg/Saar F.R.G.;2. Département de Pharmacologie, Centre Médical Universitaire, CH-1211 Genève 4 Switzerland |
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| Abstract: | By fusing liposomes which contain in the mean only one pump unit (one intramembranous particle) to planar bilayers, and provoking the ouabain-blockable leakage conductance by the presence of n-decane, the predominant unit leakage conductance associated with one pump unit was estimated to be 40–50 pS, indicating the channel nature of the leakage pathway. |
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| Keywords: | Membrane reconstitution Leakage channel Trypsin treatment n-Decane |
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