The effect of calcium on the bilayer stability of lipids from bovine rod outer segment disk membranes |
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Authors: | Arlene D. Albert Arindam Sen Philip L. Yeagle |
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Affiliation: | 1. Biochemistry Department, State University of New York at Buffalo, Buffalo NY 14214 U.S.A.;2. Biophysics Department, Roswell Park Memorial Institute, Buffalo, NY 14263 U.S.A. |
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Abstract: | The phase behavior of bovine rod outer segment disk lipids has been investigated using freeze-fracture and 31P nuclear magnetic resonance (NMR) techniques. 31P-NMR spectra of isolated disk membranes were taken as a function of temperature between 25°C and 45°C. The 31P-NMR spectrum characteristic of phospholipid bilayers was observed at all temperatures both in the absence of Ca2+ and in the presence of 10 mM and 50 mM Ca2+. A similar study was performed on lipids isolated from the disk membranes. In the absence of Ca2+ only lamellar phase behavior was observed. In the presence of less than 10 mM Ca2+, however, there was a change in morphology to non-lamellar structures. Removal of the Ca2+ caused the system to reassume the lamellar form. |
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Keywords: | Phospholipid bilayer Phase behavior Rod outer segment membrane Freeze-fracture (Bovine retina) Hepes 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid EGTA |
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