Pyrophosphate as a ligand for delivery of iron to isolated rat-liver mitochondria |
| |
Authors: | Turid Nilsen Inge Romslo |
| |
Institution: | Department of Clinical Chemistry, University of Trondheim, N-7000 Trondheim Norway |
| |
Abstract: | Rat liver mitochondria accumulate iron mobilized from transferrin by pyrophosphate. The capacity of the mitochondria to accumulate iron is higher than the capacity of pyrophosphate to mobilize iron from transferrin: with ferric-iron-pyrophosphate as iron donor, iron uptake and heme synthesis are about 10-times that at corresponding concentrations of iron-transferrin plus pyrophosphate. Uptake of iron from ferric-iron-pyrophosphate depends on a functionary respiratory chain and involves reductive cleavage of the ferric-iron-pyrophosphate complex. Apotransferrin inhibits uptake of iron from ferric-iron-pyrophosphate by competing with the mitochondria for iron. The results focus on pyrophosphate as a possible candidate for intracellular iron transport. |
| |
Keywords: | Iron Pyrophosphate Mitochondria Transferrin Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid |
本文献已被 ScienceDirect 等数据库收录! |