Derepression of the high-affinity phosphate uptake in the yeast Saccharomyces cerevisiae |
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Authors: | B.J.W.M. Nieuwenhuis G.W.F.H. Borst-Pauwels |
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Affiliation: | Department of Chemical Cytology, University of Nijmegen, Toernooiveld, Nijmegen Netherlands |
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Abstract: | Phosphate starvation derepresses a high-affinity phosphate uptake system in Saccharomyces cerevisiae strain A294, while in the same time the low-affinity phosphate uptake system disappears. The protein synthesis inhibitor cycloheximide prevents the derepression, but has no effect as soon as the high-affinity system is fully derepressed. Two other protein synthesis inhibitors, lomofungin and 8-hydroxyquinoline, were found to interfere also with the low-affinity system and with Rb+ uptake. After incubation of the yeast cells in the presence of phosphate the high-affinity system is not derepressed, but the of the low-affinity system has decreased for about 35%. Phosphate supplement after derepression causes the high-affinity system to disappear to a certain extent while in the meantime the low-affinity system reappears. The results are compared with those found in the yeast Candida tropicalis for phosphate uptake. |
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Keywords: | Phosphate transport High-affinity phosphate uptake Ion transport Derepression (S. cerevisiae) |
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