Characterization of calciphorin,the low molecular weight calciu,ionophore, from rat liver mitochondria |
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| Authors: | Indu S. Ambudkar Peter E. Kima Adil E. Shamoo |
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| Affiliation: | Membrane Biochemistry Research Laboratory, Department of Biological Chemistry, University of Maryland, School of Medicine, 660 West Redwood Street, Baltimore, MD 21201 U.S.A. |
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| Abstract: | Calciphorin, the putative mitochondrial calcium ionophore from rat liver mitochondria, exhibits the inherent properties of the mitochondrial calcium transport system and is similar to the calf heart preparation reported earlier. The protein has a strong selectivity for Ca2+, and has a Kd for Ca2+ of 56.5 ± 6.6 μM and 13.9 ± 2.1 μM in organic extraction and flow dialysis experiments, respectively. Reduction of the contaminating lipids from 23 ± 6.5 to 1.73 ± 0. moles per mole protein does not alter the affinities, Ca2+/protein soichiometry or selectivity for Ca2+. |
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| Keywords: | Calciphorin Mitochondrial membrane (Rat liver) |
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