Diazonium modification of Photosystem I. A specific effect on iron-sulfur Center B |
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Authors: | Richard Malkin |
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Institution: | Division of Molecular Plant Biology, 313 Hilgard Hall, University of California, Berkeley, CA 94720 U.S.A. |
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Abstract: | Modification of chloroplast membranes with diazonium benzene sulfonate (DABS) leads to a loss of Photosystem I-dependent ferredoxin reduction but not methyl viologen reduction. EPR studies of DABS-modified membranes show no inhibition of P-700+ formation at cryogenic temperatures, but iron-sulfur Center A photoreduction is markedly inhibited. Iron-sulfur Center B photoreduction at physiological temperatures in DABS-modified membranes is also markedly inhibited and little Center B can be detected after dark chemical reduction. These results indicate DABS specifically modifies iron-sulfur Center B of the spinach chloroplast Photosystem I electron acceptor complex and that Center B is obligately required for the reduction of Center A at cryogenic temperatures. Possible electron transport pathways at physiological temperatures are also considered. |
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Keywords: | Photosystem I Iron-sulfur center P-700 Electron transport Diazonium benzene sulfonate Chloroplast membrane DABS diazonium benzene sulfonate PSI II Photosystem I II DCIP dichlorophenol indophenol |
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