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A new method for reconstitution of highly fusogenic sendai virus envelopes |
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| Authors: | Alexander Vainstein Maty Hershkovitz Shoshana Israel Sophia Rabin Abraham Loyter |
| Affiliation: | Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem Israel |
| Abstract: | A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes. |
| Keywords: | Virus envelope Membrane reconstitution Membrane fusion (Sendai virus) PMSF phenylmethylsulfonyl fluoride TPCK To whom all correspondence should be addressed. |
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