Effects of molten-salt/ionic-liquid mixture on extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101 |
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Authors: | Sun-A Choi Joo-Young Jung Kyochan Kim Jong-Hee Kwon Jin-Suk Lee Seung Wook Kim Ji-Yeon Park Ji-Won Yang |
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Institution: | 1. Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 305-343, Korea 2. Department of Chemical and Biological Engineering, Korea University, Seoul, 136-701, Korea 3. Advanced Biomass R&D Center, KAIST, Daejeon, 305-701, Korea 4. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Korea
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Abstract: | In this study, lipid extraction from Aurantiochytrium sp. was performed using a molten-salt/ionic-liquid mixture. The total fatty acid content of Aurantiochytrium sp. was 478.8 mg/g cell, from which 145 mg/g cell (30.3 % of total fatty acids) of docosahexaenoic acid (DHA) was obtained. FeCl3·6H2O showed a high lipid extraction yield (207.9 mg/g cell), when compared with that of Emim]OAc, which was only 118.1 mg/g cell; notably however, when FeCl3·6H2O was mixed with Emim]OAc (5:1, w/w), the yield was increased to 478.6 mg/g cell. When lipid was extracted by the FeCl3·6H2O/Emim]OAc mixture at a 5:1 (w/w) blending ratio under 90 °C, 30 min reaction conditions, the fatty acid content of the extracted lipid was a high purity 997.7 mg/g lipid, with most of the DHA having been extracted (30.2 % of total fatty acids). Overall, lipid extraction from Aurantiochytrium sp. was enhanced by the synergistic effects of the molten-salt/ionic-liquid mixture with different ions. |
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