糖代谢相关酶在灵武长枣叶片和果柄糖积累中的作用

以不同发育时期灵武长枣为试材,测定果实生长发育过程中叶片、果柄可溶性糖含量及蔗糖代谢相关酶活性的变化,探讨果实生长发育过程中叶片、果柄糖的积累与蔗糖代谢相关酶活性的关系。结果表明:(1)灵武长枣叶片、果柄均主要以积累蔗糖为主,叶片、果柄中葡萄糖和果糖含量的变化平缓且随果实发育略有上升,蔗糖含量则呈先下降后迅速上升的趋势,且蔗糖含量始终高于葡萄糖和果糖的含量。(2)在果实的整个发育期,叶片和果柄的酸性转化酶(AI)活性均远高于中性转化酶(NI),AI在前期升高后变化较平稳,而蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性的变化各不相同。(3)SS分解方向酶活性(SSd)对叶片和果柄蔗糖的积累具有重要的调节作用。研究认为,蔗糖合成酶分解方向酶活性(SSd)对灵武长枣叶片和果柄蔗糖的积累起主要的调控作用。

Roles of Sucrose-metabolizing Enzymes in Sugar Accumulation in Leaves and Carpopodiums of Ziziphus jujuba Mill.cv.Lingwuchangzao

Selecting Ziziphus jujuba Mill.cv.Lingwuchangzao as materials,we determined the changes of the soluble sugar contents and the activities of sucrose metabolism-related enzymes during the development of leaf and carpopodium to study the relationship between the sugar accumulation and its metabolizing enzymes in leaf and carpopodium during the development of fruit.The result showed that:(1)The leaf and carpopodium all mainly accumulated sucrose;the contents of glucose and fructose in leaf and carpopodium kept stable and increased slightly with fruit development,but the contents of sucrose decreased at early stage and increased quickly later.The content of sucrose was higher than that of glucose and fructose all the time.(2)The acid invertase(AI) activity in leaf and carpopodium was significantly higher than that of the neutral invertase(NI) during the whole growth development period of fruit.AI activity increased at early stage and kept stable at later stage,but the change trend of the activities of sucrose synthase(SS) and sucrose phosphate synthase(SPS) was different.(3)The sucrose synthase activity in the sucrose decomposition direction(SSd) plays important regulation role in accumulating sucrose in leaf and carpopodium.To sum up,the SSd plays important regulative role in accumulating sucrose in leaf and carpopodium.