| AtWRKY63基因过表达拟南芥对核盘菌抗性的研究 |
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| 引用本文： | 陈晓婷,刘 军,林桂芳,孙翠霞.AtWRKY63基因过表达拟南芥对核盘菌抗性的研究[J].西北植物学报,2014,34(9):1721-1726. |
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| 作者姓名： | 陈晓婷 刘 军 林桂芳 孙翠霞 |
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| 作者单位： | (1 福建农林大学 生命科学学院,福州 350002;2 福建农林大学 生物农药与化学生物学教育部重点实验室,福州 350002) |
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| 基金项目： | 国家自然科学基金(30671347);福建省自然科学基金(2012J01079) |
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| 摘 要： | 为研究草酸在核盘菌致病过程中可能的作用,以模式植物拟南芥为材料,采用30mmol/L草酸喷施3周龄拟南芥,发现草酸显著诱导拟南芥AtWRKY63的表达。通过构建AtWRKY63过表达载体转化拟南芥,获得过表达AtWRKY63的纯系转基因植株,再用核盘菌活体接种拟南芥,结果表明过表达AtWRKY63植株对核盘菌的抗性显著增强。组织化学染色结果表明,AtWRKY63是通过诱导植物的氧爆发,抑制核盘菌菌丝的生长来抵御核盘菌的侵染;qRT-PCR对拟南芥转录水平分析表明,AtWRKY63可能激活了过表达植株的水杨酸与茉莉酸依赖的抗病信号途径,从而增强对核盘菌的抗性。
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| 关 键 词： | 拟南芥 AtWRKY 核盘菌 草酸 |
AtWRKY63-overexpression in Arabidopsis Confers Resistance to Sclerotinia sclerotiorum |
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| CHEN Xiaoting,LIU Jun,LIN Guifang,SUN Cuixia.AtWRKY63-overexpression in Arabidopsis Confers Resistance to Sclerotinia sclerotiorum[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(9):1721-1726. |
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| Authors: | CHEN Xiaoting LIU Jun LIN Guifang SUN Cuixia |
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| Abstract: | To explore the potential roles of oxalic acid (OA) in the pathogenesis of Sclerotina sclerotiorum,we sprayed 30 mmol/L OA evenly onto 3-week-old Arabidopsis and found AtWRKY63 was markedly induced by OA.To further elucidate the function of AtWRKY63,we obtained pure line of AtWRKY63-overexpression Arabidopsis by constructing AtWRKY63-overexpression vector and transforming Arabidopsis.The Arabidopsis plants overexpressing AtWRKY63 showed enhanced resistance to S.sclerotiorum.Histochemical staining revealed that overexpression of AtWRKY63 induced oxidative burst in host plants,which suppressed the hyphal growth of S.sclerotiorum,and consequently inhibited fungal infection.qRT-PCR analysis indicated that AtWRKY63 may involve in salicylic acid-dependent and jasmonic acid-dependent defense signaling pathway,thus,confers the resistance to S.sclerotiorum. |
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| Keywords: | Arabidopsis thaliana AtWRKY63 Sclerotinia sclerotiorum oxalic acid |
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