Site-specific integration of an F' lac pro factor in the region of the replication origin (oriC) of E. coli |
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| Authors: | Laurie Simon LaVerne and Dan S. Ray |
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| Affiliation: | (1) Molecular Biology Institute and Department of Biology, University of California, 90024 Los Angeles, California, USA |
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| Abstract: | Summary An episome, F 128, which carries approximately 8x104 base pairs of chromosomal DNA homologous to the lac pro region of the E. coli chromosome, has been found to integrate into the oriC region of the chromosome in a site specific reaction. While the event appears to be recA-dependent, no homology between the episome and this region of the chromosome was detected. The Hfr strains formed result from the integration of intact F 128 molecules. The structure of the Hfr strains generated has been determined and their transfer properties analyzed. |
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