| 扩增共有序列遗传标记(ACGM)及其在植物中的应用价值 |
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| 引用本文： | 何俊平,阮松林,祝水金,马华升. 扩增共有序列遗传标记(ACGM)及其在植物中的应用价值[J]. 遗传, 2009, 31(9): 913-920. DOI: 10.3724/SP.J.1005.2009.00913 |
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| 作者姓名： | 何俊平 阮松林 祝水金 马华升 |
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| 作者单位： | 1. 浙江大学农业与生物技术学院, 杭州 310029;2. 杭州市农业科学研究院生物技术研究所, 杭州 310024 ; |
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| 基金项目： | 中国博士后科学基金资助项目 |
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| 摘 要： | 扩增共有序列遗传标记(Amplified consensus genetic markers, ACGM)是建立在亲缘关系较近的物种间同源基因中编码序列(外显子)存在高度保守性, 而非编码区(内含子)存在潜在多态性基础之上的一种基于PCR的新型DNA分子标记技术。随着比较基因组学和生物信息学的迅猛发展, ACGM技术已成为物种间同源基因比较、系统发生关系分析和感兴趣基因定位的有效工具。文章就ACGM技术尤其是它在芸薹属和禾本科物种中的应用研究做详细介绍。同时, 对ACGM技术可能的应用前景进行了展望。
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| 关 键 词： | ACGM 基因组进化 基因定位 |
| 收稿时间： | 2008-12-26 |
| 修稿时间： | 2009-03-30 |
Amplified consensus genetic markers and its application in plants |
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| HE Jun-Ping,RUAN Song-Lin,ZHU Shui-Jin,MA Hua-Sheng. Amplified consensus genetic markers and its application in plants[J]. Hereditas, 2009, 31(9): 913-920. DOI: 10.3724/SP.J.1005.2009.00913 |
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| Authors: | HE Jun-Ping RUAN Song-Lin ZHU Shui-Jin MA Hua-Sheng |
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| Affiliation: | 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Institute of Biotechnology, Hangzhou Academy of Agricultural Science, Hangzhou 310024, China ; |
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| Abstract: | Amplified consensus genetic markers (ACGM) is a novel PCR-based DNA molecular marker technique, which is based on the conservation of coding sequences and the potential polymorphism within non-coding sequences in homologous genes among closely related species. Along with the rapid development of comparative genomics and bioinformatics, the ACGM technique has already become a powerful tool for comparing homologous genes, analyzing phylogenetic relationships among species, and mapping of genes of interest. Here, an introduction to ACGM technique, especially its application in Brassica genera and Gramineae, was presented in detail. The prospects of ACGM were also discussed. |
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| Keywords: | ACGM |
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