Osteonectin-derived peptide increases the modulus of a bone-mimetic nanocomposite |
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Authors: | Alireza S Sarvestani Xuezhong He Esmaiel Jabbari |
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Institution: | (1) Biomimetic Materials and Tissue Engineering Laboratories, Department of Chemical Engineering, Swearingen Engineering Center, University of South Carolina, Rm 2C11, Columbia, SC 29208, USA |
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Abstract: | Many factors contribute to the toughness of bone including the presence of nano-size apatite crystals, a dense network of
collagen fibers, and acidic proteins with the ability to link the mineral phase to the gelatinous collagen phase. We investigated
the effect of a glutamic acid (negatively charged) peptide (Glu6), which mimics the terminal region of the osteonectin glycoprotein
of bone, on the shear modulus of a synthetic hydrogel/apatite nanocomposite. One end of the synthesized peptide was functionalized
with an acrylate group (Ac-Glu6) to covalently attach the peptide to the hydrogel phase of the composite matrix. When microapatite
crystals (5 μm diameter) were used, addition of Ac-Glu6 peptide did not affect the modulus of the microcomposite. However,
when nanoapatite crystals (100 nm diameter) were used, addition of Ac-Glu6 resulted in significant reinforcement of the shear
modulus of the nanocomposite (∼100% in elastic shear modulus). Furthermore, addition of Ac-Gly6 (a neutral glycine sequence)
or Ac-Lys6 (a positively charged sequence) did not reinforce the nanocomposite. These results demonstrate that the reinforcement
effect of the Glu6 peptide, a sequence in the terminal region of osteonectin, is modulated by the size of the apatite crystals.
The findings of this work can be used to develop advanced biomimetic composites for skeletal tissue regeneration. |
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Keywords: | Nanocomposite Hydrogel Apatite Peptide reinforced Bone |
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