Fluorescent monitoring of cellular physiological status depending on the accumulation of ppGpp |
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Authors: | Hisakage Funabashi Masayasu Mie Yasuko Yanagida Eiry Kobatake Masuo Aizawa |
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Affiliation: | (1) Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan |
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Abstract: | For evaluating the physiological status of cells, astringent response network was used. Fluorescence from intact E. coli, which has a plasmid encoding the green fluorescence protein (GFP) under the regulation of rpoS promoter, was monitored. Comparison of the response of different E. coli strains demonstrated an essential role of ppGpp in the expression of GFP, as it activated the rpoS promoter. The physiological status of intact cells, that depends on ppGpp accumulation in response to the nutritional status such as amino acid starvation, could therefore be monitored by measuring fluorescent intensity using this reporter gene. |
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Keywords: | cellular physiological status non-destructive monitoring ppGpp rpoS promoter stringent respons |
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