Rhizopus delemar Lipase in Microemulsion-based Organogels: Reactivity and Rate-Limiting Study |
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| Authors: | Kazuhito Nagayama Kaori Tada Kazumitsu Naoe Masanao Imai |
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| Affiliation: | a Department of Materials Science and Engineering, Kochi National College of Technology, Japanb Department of Chemical Engineering, Nara National College of Technology, Japanc Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa |
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| Abstract: | The reactivity of Rhizopus delemar lipase immobilized in sodium bis(2-ethylhexyl) sulfosuccinate microemulsion-based organogels (MBGs) was investigated as a function of the water content in the gel. Gelatin was used as the gelling component of the MBGs. The maximal reaction rate of 6.5 μM s-1 was obtained at a WG (molar ratio of water to AOT in the MBG phase) value of 100. In the experimental WG conditions, the reaction proceeded under an effectiveness factor of 0.02 to 0.04 giving the reaction-limiting regime. |
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| Keywords: | AOT Gelatin Organogels Lipase Esterification Rate-limiting |
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