Investigations on the PGM (phosphoglucomutase) polymorphism by isoelectric focusing inDrosophila melanogaster |
| |
Authors: | Rosadele Cicchetti Gabriella Argentin Benedetto Nicoletti |
| |
Affiliation: | (1) Dip.nto di Sanità Pubblica e Biologia cellulare, Università di Tor Vergata, Roma, Italy |
| |
Abstract: | Pgm allele frequencies of 383 individuals were determined in a sample ofDrosophila melanogaster from three laboratory Sardinian populations, using the techniques of standard electrophoresis, heat denaturation, and isoelectric focusing. The analysis of the progeny obtained from informative crosses showed that the isoelectric focusing patterns segregate in a Mendelian way. ThePgm 1.00 andPgm 0.70 electrophoretic alleles displayed different isoelectric points, whereas thePgm 1.00,tr andPgm 1.00,ts isoelectrophoretic alleles could not be differentiated when tested by isoelectric focusing. Moreover, thePgm 0.70,ts allele was split into two classes, with isoelectric points ofpH 6.4 andpH 6.6. |
| |
Keywords: | Drosophila melanogaster phosphoglucomutase nonelectrophoretic variability isoelectric focusing |
本文献已被 SpringerLink 等数据库收录! |
|