Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1 |
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| Authors: | M. A. Burkin I. V. Yakovleva V. V. Sviridov A. A. Burkin G. P. Kononenko N. A. Soboleva |
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| Affiliation: | (1) Present address: Russian Academy of Medical Sciences, Mechnikov Institute of Vaccines and Sera, 103064 Moscow, Russia;(2) Present address: Hygiene, and Ecology, Russian Academy of Agricultural Sciences, All-Russia Institute of Veterinary Sanitation, 123022 Moscow, Russia |
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| Abstract: | Four hybrid clones (MM-(AB1)-1, MM-(AB1)-2, MM-(AB1)-3, and MM-(AB1)-4) were obtained by hybridoma technology involving the immunization of BALB/c mice with a BSA conjugate of aflatoxin B1 carboxymethyloxime derivative. Antibodies produced by these clones varied in their ability to recognize the aflatoxin B1 analogues. The sensitivity of enzyme immunoassay based on all monoclonal antibodies was higher compared to analysis based on polyclonal rabbit antibodies (0.1 and 0.4 ng/ml, respectively). |
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