Hypoxia enhances prostaglandin synthesis in renal mesangial cell cultures |
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Authors: | Wolfgang Jelkmann Armin Kurtz Ulrich Frstermann Josef Pfeilschifter Christian Bauer |
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Institution: | 2. Institut für Physiologie der Universität, Universitätsstraße 31, D-8400 Regensburg, FRG;3. Pharmakologisches Institut der Universität, Hermann-Herder-Straße 5, D-7800 Freiburg, FRG |
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Abstract: | In view of recent findings which suggest that renal prostaglandins mediate the effect of hypoxia on erythropoietin production, we have studied whether hypoxia is a stimulus for in vitro prostaglandin synthesis. Studies were carried out in rat renal mesangial cell cultures which produce erythropoietin in an oxygen-dependent manner. Production rates of PGE2 and in specified samples also of 6-keto-PGF1α, as a measure of PGI2, and PGF2α were determined by radioimmunoassay after incubation at either 20% O2 (normoxic) or 2% O2 (hypoxic) in gas permeable dishes for 24 hrs. Considerable variation in PGE2 production was noted among independent cell lines. PGE2 production appeared to be inversely correlated to the cellular density of the cultures. In addition, PGE2 production was enhanced in hypoxic cell cultures. The mean increase was 50 to 60%. PGF2α and 6-keto-PGF1α increased by about the same rate. These results indicate that hypoxia is a stimulus for in vitro prostaglandin production. |
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Keywords: | Reprint address: Dr Wolfgang Jelkmann Institut für Physiologie MHL Ratzeburger Allee 160 D-2400 Lübeck FRG |
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