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The aluminium(III)-sialic acid interaction: A potential role in aluminium-induced cellular membrane degeneration |
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| Authors: | V.B. Di Marco A. Venzo R. Seraglia |
| Affiliation: | a Department of Chemical Sciences, University of Padova, via Marzolo 1, 35131 Padova, Italy b Bioinorganic Chemistry Research Group of the Hungarian Academy of Sciences, University of Szeged, P.O. Box 440, H-6701 Szeged, Hungary c CNR, Institute of Sciences and Molecular Technologies, via Marzolo 1, 35131 Padova, Italy d Department of Chemical Processes of Engineering, University of Padova, via Marzolo 9, 35131 Padova, Italy e CNR, Institute of Sciences and Molecular Technologies, Corso Stati Uniti 4, 35100 Padova, Italy f Department of Inorganic and Analytical Chemistry, University of Szeged, P.O. Box 440, H-6701 Szeged, Hungary |
| Abstract: | The coordination between Al(III) and sialic acid (N-acetylneuraminic acid, HL, pKa = 2.58 ± 0.01) was studied by potentiometric titrations at 25 °C in aqueous 0.2 M KCl, by 1H NMR, and by electrospray ionization mass spectrometry (ESI-MS). The potentiometric measurements gave the following aluminium complex stoichiometries and stability constants:  , log β(AlLH−2) = −6.34 ± 0.02, and log β(AlL2H−1) = −1.14 ± 0.04. The 1H NMR spectra yielded structural information on species  . The ESI-MS data confirmed the metal-ligand stoichiometry of the complexes.The metal-ligand speciation at micromolar Al(III) concentrations (i.e., under in vivo conditions) at physiological pH values reveals that considerable amount of Al(III) is complexed. This suggests that the toxic effect of Al(III) towards cellular membranes might be due to its coordination by protein-bound sialic acid. |
| Keywords: | Al(III) Aluminium toxicity Sialic acid Potentiometry, NMR ESI-MS |
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