Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins |
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| Authors: | Julie A. McKenzie Robert L. Raison Donald E. Rivett |
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| Affiliation: | (1) Clinical Immunology Research Centre, University of Sydney, 2006 Sydney, New South Wales, Australia;(2) Division of Protein Chemistry, CSIRO, 3052 Parkville, Australia |
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| Abstract: | A new protein crosslinking agent, 2,3-dibromopropionyl-N-hydroxysuccinimide ester, has been synthesized and characterized. The potential use of this compound as a temperature-controllable heterobifunctional crosslinking agent has been investigated using model systems and its reactivity compared with that of chlorambucil-N-hydroxysuccinimide ester. The coupling of14C-labeled phenylethylamine to lysozyme has been used to illustrate the feasibility of the use of this crosslinking agent for the synthesis of immunotoxins. |
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| Keywords: | immunotoxins bifunctional crosslinking agents protein conjugation |
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