pSEUDO, a Genetic Integration Standard for Lactococcus lactis
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| Authors: | Joao P. C. Pinto Araz Zeyniyev Harma Karsens Hein Trip Juke S. Lolkema Oscar P. Kuipers Jan Kok |
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| Affiliation: | 1Departments of Molecular Genetics;2Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands |
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| Abstract: | Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and two general stress reporter strains (NZ9000::PhrcA-GFP and NZ9000::PgroES-GFP) enabling in vivo noninvasive monitoring of cellular fitness were constructed. |
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