枳壳外植体离体再生及农杆菌介导的遗传转化

以枳壳实生苗的上胚轴及茎段为材料，在附加有ＢＡ和ＭＴ培养基上进行培养，上胚轴出芽率普遍高于茎段，ＢＡ为１ｍｇ／Ｌ时，出芽率最高，ＢＡ浓度升高，出芽率随之下降。外植体在培养基上的接种方式，对出芽有一定影响，上胚轴切段 形态学下端垂直插入，出芽率高。

In Vitro Culture of Explants and the Agrobacterium Mediated Genetic Transformation of Poncirus trifoliata

Epicotyls and stem segments from sterile seeding of Poncirus trifoliata Raf. Were cultured in vitro. The results indicated that epicotyls were suitable for culture in vitro, BA at a concentration of 1 mg/L gave the highest shoot formation frequency (86 0%). As BA concentrations increased, the ability of shoot formation decreased. Epicotyls oriented with their apical ends protruding from the medium produced more shoots than when they were placed with their basal end upright or were placed horizontally. Explants used for transformation were the epicotyls from Poncirus trifoliata Raf. The selection pressure for kanamycin was 50mg/L. Ceftaxime used as antibiotics was better than carbenicillin. After 1 5 months 14 2% resistant shoots were emerged from the explants. Histochemical GUS assay showed that 70 0% of the resistant plants were GUS positive.