Adenosine effects on hormone-stimulated steroidogenesis in isolated bovine adrenal zona fasciculata cells |
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Authors: | W J Koper S J Yeaman R C Honnor |
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Institution: | Department of Biochemistry, University of Newcastle-upon-Tyne, England. |
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Abstract: | In isolated bovine adrenal zona fasciculata cells, the use of adenosine deaminase to remove endogenous adenosine had no effect on basal or angiotensin II-stimulated steroidogenesis but enhanced ACTH1-24-stimulated steroidogenesis over the entire dose response range without appreciable change in potency of ACTH1-24. 8-Phenyl-theophylline, an adenosine antagonist, mimicked all of the actions of adenosine deaminase. High concentrations (greater than 1 microM) of N6-phenylisopropyl-adenosine (PIA) increased basal, angiotensin II and cyclic AMP-stimulated steroidogenesis, whilst inhibiting the ACTH1-24-stimulated condition. PIA also increased the potency of angiotensin II approx 20-fold. These observations are consistent with the possibility that adenosine exerts effects on two different signalling systems within zona fasciculata cells. |
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