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A centralized model for creating shared,standardized, microsatellite data that simplifies inter-laboratory collaboration |
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| Authors: | Jeff J. Stephenson Matt R. Campbell Jon E. Hess Chris Kozfkay Andrew P. Matala Megan V. McPhee Paul Moran Shawn R. Narum Melanie M. Paquin Ora Schlei Maureen P. Small Donald M. Van Doornik John K. Wenburg |
| Affiliation: | 1. Columbia River Inter-Tribal Fish Commission, Hagerman Fish Culture Experiment Station, 3059-F National Fish Hatchery Road, Hagerman, ID, 83332, USA 2. Idaho Department of Fish and Game, 1800 Trout Road, Eagle, ID, 83616, USA 3. Conservation Biology Division, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, Seattle, WA, 98112, USA 4. Abernathy Fish Technology Center, U.S. Fish & Wildlife Service-Abernathy, 1440 Abernathy Creek Road, Longview, WA, 98632, USA 5. Columbia River Inter-Tribal Fish Commission, Hagerman Fish Culture Experiment Station, 3059-F National Fish Hatchery Road, Hagerman, ID, 83332, USA 6. Flathead Lake Biological Station, University of Montana, 32125 Biological Station Lane, Polson, MT, 59860, USA 7. Conservation Genetics Laboratory, Alaska Region, U.S. Fish and Wildlife Service, 1011 East Tudor Road, Anchorage, AK, 99503, USA 8. Fish Program, Conservation Biology Unit, Science Division, Washington Department of Fish and Wildlife, 600 Capital Way N., Olympia, WA, 98501-1091, USA 9. NOAA Fisheries, Manchester Research Station, 7305 Beach Drive East, Port Orchard, WA, 98366, USA |
| Abstract: | We demonstrate an efficient model for standardizing microsatellite DNA data among laboratories studying Oncorhynchus mykiss. Eight laboratories standardized 13 microsatellite loci following allele nomenclature of a central laboratory (average inter-laboratory genotyping concordance >98%). Following this central model, we have currently standardized 298 alleles from throughout the species native range. Although we focus here on O. mykiss, our experiences and recommendation apply equally to other broadly distributed species that may benefit from multi-laboratory collaborative data collection. |
| Keywords: | Microsatellites Standardization Steelhead Oncorhynchus mykiss |
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