Kinetic study of racemization of aspartyl residues in synthetic elastin peptides |
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Authors: | K Kuge N Fujii Y Miura S Tajima T Saito |
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Institution: | (1) Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka, Japan;(2) Department of Dermatology, National Defense Medical College, Namiki, Tokorozawa, Saitama, Japan |
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Abstract: | Summary. We previously reported that biologically uncommon D-aspartyl residues are present in sun-damaged skin from elderly people, possibly in elastin. Here, we report the kinetics of Asp racemization in model peptides corresponding to elastin sequences from exons 6 and 26. We estimated the activation energy (E) of racemization of Asp residues, the racemization rates (RR) at 37°C and the time (t) required for the D/L ratio of Asp to approximate to 1.0 (D/L ratio of Asp=0.99) at 37°C. For an exon 6 peptide, E=29.0 kcal/mol, RR=2.59×10–2/yr and t=101.0 yr. For an exon 26A peptide E=26.2 kcal/mol, RR=4.27×10–2/yr and t=61.3 yr; and for a second exon 26A peptide E=25.7 kcal/mol, RR=5.55×10–2/yr and t=47.0 yr. These results suggest that racemization of Asp residues in elastin could occur within a human life span. We propose that D-Asp could be a useful molecular indicators of aging. |
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Keywords: | : Aging – D-Aspartic acid – Elastin – Kinetics – Racemization – Racemization rate constant – Skin |
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