Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.) |
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| Authors: | G M Timmerman T J Frew N F Weeden A L Miller D S Goulden |
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| Institution: | (1) New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand;(2) Department of Horticultural Science, Cornell University, 14456 Geneva, NY, USA;(3) Present address: Molecular Pathology Department, Christchurch Clinical School of Medicine, Christchurch Hospital, Christchurch, New Zealand |
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| Abstract: | Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR). |
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| Keywords: | Pea Powdery mildew Genome mapping Molecular markers Bulked segregant analysis |
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