Isolation of myoinositol from solutions of enzymatic biotransformation |
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Authors: | Reut E N Rakhimov M M |
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Institution: | Mirzo Ulugbek Uzbekistan National University, Biological and Soil Department, Tashkent, 700174 Uzbekistan. |
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Abstract: | The transferase reaction between phospholipids and inositol catalyzed by phospholipase D on phase interface in water-organic solvent systems was studied. Optimal conditions for phosphatidylinositol synthesis in water-organic solvent heterogeneous system were determined. The rapid separation of the hydrophobic components, phospholipids, from water-soluble products, alcohols, was observed in the systems with organic solvents. Displacement of myo-inositol from phosphatidylinositol by methanol, alcohol substrate, added to the reaction medium was shown in hexane-water system. Myo-inositol was isolated from the mixture of its isomers by two-stage transferase reaction catalyzed by phospholipase D. |
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