Two-dimensional electrophoresis of the plasma proteins of alpacas and llamas: genetic polymorphism of α1B-glycoprotein and three other proteins |
| |
Authors: | R. K. JUNEJA,M. C.T. PENEDO,H. E. B. LARSSON&dagger ,B. GAHNE,A. T. BOWLING |
| |
Affiliation: | Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden;*Serology Laboratory, School of Veterinary Medicine, University of California, Davis, California, USA;†Department of Genetics, University of Uppsala, Uppsala, Sweden |
| |
Abstract: | Summary. Plasma samples of alpacas and llamas were analysed by a simple method of two-dimensional (2-D) agarose gel (pH 8.6)-horizontal polyacrylamide gel (pH 9.0) electrophoresis, followed by general protein staining of gels. Genetic polymorphism in both species is described for α 1 B-glycoprotein (α 1 B) and three other unidentified proteins designated prealbumin (Pr), postalbumin 1 and 2 (Pal and Pa2). α 1 B was identified by cross-reactivity with antisera for human and pig α 1 B. Altogether, two alleles of Pr, two of Pa1, five of α 1 B and three of Pa2 are described. Most of the alleles were present in alpacas and llamas. Alpacas showed a high degree of polymorphism at all four loci. Llamas showed considerable polymorphism at only the Pa1 and Pa2 loci. The theoretical probability of exclusion (P e ) of an incorrectly assigned parent was estimated to be about 80% in each species by typing for the six polymorphic plasma proteins reported so far in these species. The given method of 2-D electrophoresis revealed no fixed differences in protein mobilities that discriminate between llamas and alpacas. |
| |
Keywords: | alpaca llama plasma proteins α1B-glycoprotein genetic polymorphism immunoblotting camelids |
|
|