| 转座子Tn917在短小芽孢杆菌中的转座作用 |
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| 引用本文： | 耿运琪 蒋如璋. 转座子Tn917在短小芽孢杆菌中的转座作用[J]. 微生物学报, 1992, 32(4): 305-307 |
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| 作者姓名： | 耿运琪 蒋如璋 |
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| 作者单位： | 南开大学生物系生物工程研究室 天津 300071 |
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| 摘 要： |
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| 关 键 词： | 转座子 短小芽孢杆菌 转座作用 |
Transposition of Tn917 in Bacillus pumilus] |
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| Y Geng,R Jiang. Transposition of Tn917 in Bacillus pumilus][J]. Acta microbiologica Sinica, 1992, 32(4): 305-307 |
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| Authors: | Y Geng R Jiang |
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| Affiliation: | Department of Biology, Nankai University, Tianjin. |
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| Abstract: | Transposition Tn917 was introduced into Bacillus pumilus 289 by protoplast transformation with plasmid pTV32. The temperature-sensitive replication property of pTV32 was maintained in B. pumilus. Tn917 was transposed efficiently in B. pumilus with 4.8 x 10(-4) transposition rate. The yield of auxotrophs was about 0.65% in all insertional mutants. It indicated a prospects for the use of Tn917 as a tool for insertional mutagenesis and genetic manipulation in B. pumilus. |
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