枯芽孢杆菌α—淀粉酶基在在大肠杆菌中的表达及其产物的分泌

Expression and secretion of alpha-amylase gene from Bacillus subtilis in E. coli]

The E. coli which carrying the alpha-amylase gene fragment cloned from B. subtilis secreted the gene products into the medium. The reason is the exogenous gene fragment act on the cell wall of E. coli by some way, gives rise to the change of its structure. It leads up to the alpha-amylase and some periplasm proteins passing through the cell wall into the medium. It also causes the change of host colonial morphology. The secrete process are non-specific.