离体培养下番红花花柱—柱头状物再生的研究

番红花(Crocus sativus L.)幼花被和幼花柱可以诱导花柱-柱头状物的再生,长度为24mm 的花芽上分离的花被具有最高的诱导频率(37.5%);花被外植体附加 K5 mg/L 和NAA 4mg/L 有利于再生花柱-柱头状物,频率达到35.3%;附加6-BAP 7 mg/L 和 NAA9mg/L 则有利于柱头状物的大量形成。

STUDY ON IN VITRO REGENERATION OF STYLE-STIGMALIKE STRUCTURE IN CROCUS SATIVUS L.

Effects of age and variety of explant as well as of exogenous hormones on regeneration of style-stigma-like structure in vitro were observed in saffron.(1)Explants excised from stalk, perianth,anther,ovary,style and stigma showed different reactions in vitro,only young pe- rianth and style could regenerate the style-stigma-like structures;(2)Age of the perianth ex- plant had obvious effect on induction frequency,and the explants excised from an inflorescen- ce of 24mm in length showed the highest frequency(37.5%)and(3)Experiments on exoge- nous hormones made clear that supplement with K 5mg/L and NAA 4mg/L was advantageous to regeneration of style-stigma-like structure.Induction frequency reached 35.3%.Supplement with 6-BAP 7mg/L and NAA 9mg/L could promote the formation of stigma like structures.