| 赤霉烯酮的微生物还原 |
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| 引用本文： | 吴莲芬 王秋敏 王淑菊 周凤仪. 赤霉烯酮的微生物还原[J]. 微生物学报, 1992, 32(1): 11-16 |
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| 作者姓名： | 吴莲芬 王秋敏 王淑菊 周凤仪 |
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| 作者单位： | 中国科学院上海有机化学研究所 上海 200032 |
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| 摘 要： | 用Rhodtorula sp.Saccharomyces sp.Arthorbacter sp.和Candida sp．四属20株菌对由Fusarium graminearum 产生的类雌激素——赤霉烯酮(Zearalenone)的还原转化进行了研究。实验结果证明，Rhodotorula sp和 Arthrobacter sp．的还原产物主要是α-赤霉烯醇(α-Zearalenol，经HPLC鉴定含量分别为96％和84％)。Saccharomyces sp．和Candidasp.的主要还原产物是β-赤霉烯醇(β-Zearalenol，经HPLC鉴定含量分别为91％和92％)。产物均经HPLC、13C—NMR和MS鉴定确证。
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| 关 键 词： | 赤霉烯酮；赤霉烯醇 |
Biotransformation of zearalenone] |
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| L Wu,Q Wang,S Wang,F Zhou. Biotransformation of zearalenone][J]. Acta microbiologica Sinica, 1992, 32(1): 11-16 |
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| Authors: | L Wu Q Wang S Wang F Zhou |
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| Affiliation: | Shanghai Institute of Organic Chemistry, Academia Sinica. |
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| Abstract: | The conversion of Zearalenone by some strains of microorganisms was investigated. When the selective strains of Rhodotorula sp., Arthrobacter sp., Saccharomyces sp., and Candida sp. were incubated by shaking or standing at 28 degrees C for 72 h with an alcoholic solution of Zearalenone as the substrate at a concentration of 2-10 mg/ml ethanol (50-100 micrograms/ml medium), it was readily converted to give Zearalenols, consisting either mainly of the alpha-isomer (e.g. 96% in case of Rhodotorula sp. and 84% in case of Arthrobacter sp. as determined by HPLC) or beta-isomer (e.g. 91% and 92% in Saccharomyces sp. and Candida sp., respectively). The structure of the product was confirmed by 13C-NMR, MS and HPLC. |
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