Evaluation of the necessity for rapid inactivation of brain enzymes prior to analysis of norepinephrine dopamine and serotonin in the mouse. |
| |
| Authors: | S T Weintraub W B Stavinoha R L Pike W W Morgan A T Modak S H Koslow L Blank |
| |
| Institution: | 1. Department of Pharmacology, The University of Texas Health Science Center at San Antonio 78284, USA;2. Department of Chemistry, University of Oklahoma, Norman, Oklahoma 73069, USA;3. Department of Anatomy, The University of Texas Health Science Center at San Antonio 78284, USA;4. Department of Preclinical Pharmacology, NIMH, St. Elizabeth''s Hospital, Washington D.C. 20032, USA |
| |
| Abstract: | Norepinephrine, dopamine and serotonin concentrations were measured in mouse whole brain. Animals were killed either by decapitation or by exposure to 250 msec microwave irradiation which produces irreversible inactivation of brain enzymes. The biogenic amines were determined by mass fragmentometry, fluorometry and by a combination of high performance liquid chromatography and an electrochemical detector. No differences were found in the levels of these neurochemicals between the two methods of animal sacrifice. Therefore, rapid inactivation of brain enzymes is not necessary prior to analysis for catecholamines and serotonin in mouse whole brain. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
