Habituation in sugarbeet callus: auxin content, auxin protectors, peroxidase pattern and inhibitors |
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Authors: | C. Kevers M. Coumans W. De Greef M. Hofinger Th. Gaspar |
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Affiliation: | Laboratoire de Biologie vegetale, 22, quai Van Beneden, B-4020 Liege, Belgium |
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Abstract: | A GC-ECD titration of IAA in normal auxin (2,4-D)-requiring and auxin-independent (habituated) sugarbeet callus revealed an equal amount in both tissues. A comparison of the content and pattern (through starch gel electrophoresis) of soluble, membrane and wall peroxidases indicated that normal tissues contained a higher level of isoperoxidases. Normal tissues also were found to contain higher levels of peroxidase inhibitors and auxin protectors. The hypothesized peroxidase-mediated higher rate of auxin destruction in normal sugarbeet callus is supposed to be counterbalanced by the 2,4-D-controlled auxin protectors. |
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Keywords: | Sugarbeet callus habituation auxin level peroxidase |
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