Advantages in immunofluorescence of dichlortriazinylaminofluorescein conjugates isolated by single-step gel filtration]

The author describes a simple method obtaining dichlortriazinylaminofluoresceine (DCTAF) conjugates to human immunoglobulins and separation of their optimally labeled fractions. The single-stage method of the conjugate release from the nonbound flurorchrome and of a simultaneous separation of the optimally labeled fractions was carried out on Sephadex G-25 at pH 5.6--6.6 and at low ionic power of the eluate. Proteins overloaded with the dye are sorbed on gel according to their isoelectrical points.