Optimal conditions for the production of exopolysaccharide by marine microorganismHahella chejuensis |
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Authors: | Sung-Hwan Ko Hyun Sang Lee Shin Hye Park Hong Kum Lee |
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Institution: | (1) Microbiology Lab., Korean Ocean Research & Development Institute, Ansan P.O. Box 29, 425-600 Ansan, Korea |
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Abstract: | A marine microorganism, strain 96CJ10356 produced exopolysaccharide, designated as EPS-R. To optimize culture conditions for
the production of EPS-R, carbon and nitrogen sources, mineral salts, temperature, and pH were examined. From this study, STN
medium for the production of EPS-R was suggested as follows; sucrose 20 g, tryptone 10 g, NaCl 10 g, MgSO4 5 g, CaCl2 1 g, KH2PO4 76 mg, K2HPO4 83 mg, FeCl2 5 mg, MnCl2 1 mg, NaMoO4 1 mg, and ZnCl2 1 mg per liter at pH 7.0. About 9.23 g/L of EPS-R was obtained from STN medium after cultivation for 120 h at 25°C in a 5-liter
jar fermentor with an aeration rate of 0.17 vvm. Apparent viscosity and flocculation activity of the culture broth were increased
with the production of EPS-R and the maximal values were 415 cP and 1400 unit/mL against 0.5% activated carbon, respectively. |
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Keywords: | exopolysaccharide marine microorganism culture conditions Hahella chejuensis |
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