Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts |
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| Authors: | Swapan K. Datta Karabi Datta Nouchine Soltanifar Gunter Donn Ingo Potrykus |
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| Affiliation: | (1) ETH-Zurich, Institute of Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland;(2) Hoechst AG, D-6230 Frankfurt am Main 80, Germany |
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| Abstract: | The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hgr and 170 PPTr IR72 plants from protoplasts through selection. 31 Hgr and 70 PPTr plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT. |
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| Keywords: | Indica rice IRRI breeding line PEG-mediated protoplast transformation hygromycin phosphotransferase phosphinothricin acetyltransferase herbicide resistance |
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