The complex <Emphasis Type="Italic">whiJ</Emphasis> locus mediates environmentally sensitive repression of development of <Emphasis Type="Italic">Streptomyces coelicolor</Emphasis> A3(2) |
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Authors: | José A Aínsa Nick Bird N Jamie Ryding Kim C Findlay Keith F Chater |
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Institution: | 1.Department of Molecular Microbiology,John Innes Centre, Norwich Research Park, Colney,Norwich,UK;2.Departamento de Microbiología, Facultad de Medicina,Universidad de Zaragoza,Zaragoza,Spain;3.Verenium Corporation,San Diego,USA |
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Abstract: | A segment of DNA was isolated that complemented several poorly characterised sporulation-defective white-colony mutants of
Streptomyces coelicolor A3(2) from an early collection (Hopwood et al., J Gen Microbiol 61: 397–408, 1970). Complementation was attributable to a
gene, SCO4543, named whiJ, encoding a likely DNA-binding protein. Surprisingly, although some mutations in whiJ had a white colony phenotype, complete deletion of the wild-type or mutant gene gave a wild-type morphology. The whiJ gene is a member of a large paralogous set of S. coelicolor genes including abaAorfA, which regulates antibiotic production; and genes flanking whiJ are paralogues of other gene classes that are often associated with whiJ-like genes (Gehring et al., Proc Natl Acad Sci USA 97: 9642–9647, 2000). Thus, the small gene SCO4542 encodes a paralogue
of the abaAorfD gene product, and SCO4544 encodes a paralogue of a family of likely anti-sigma factors (including the product of abaAorfB). Deletion of SCO4542 resulted in a medium-dependent bald- or white-colony phenotype, which could be completely suppressed
by the simultaneous deletion of whiJ. A model is proposed in which WhiJ binds to operator sequences to repress developmental genes, with repression being released
by interaction with the WhiJ-associated SCO4542 protein. It is suggested that this activity of SCO4542 protein is prevented
by an unknown signal. |
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