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HPLC determination of glycolic acid in seawater. An estimation of phytoplankton photorespiration in the Gulf of Lions, western Mediterranean Sea
Authors:Leboulanger, Christophe   Descolas-Gros, Chantal   Jupin, Henri
Affiliation:1Laboratoire d'Hydrobiologie URA 1355, Universit de Montpellier II, CC 093, 34095 Montpellier Cedex 05, France Laboratoire de Biologie Vgtale, Universit de Perpignan 66 860 Cedex
Abstract:A method is described that allows improved quantification ofglycolic acid dissolved in seawater. The extraction of glycolicacid was performed by an organic solvent and its quantificationby HPLC. With this method, the glycolic acid dissolved in seawateris extracted in a reproducible manner with a constant yield,and dosed with a precision >7.5%. Values ranging from 24to 89 µg l–1 were measured in the open sea off Banyuls-sur-merin spring 1992, when a series of samples were taken around solarmidday on board RS ‘Nris’ during a phytoplanktonicbloom.
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